巢毅敏学术报告
报告题目：Carbohydrate capped Silicon Nanoparticles for targeting cancer cells
报告人：巢毅敏
时间：2015-3-24（周二）上午10:00-11:30
地点：逸夫科技馆三楼会议室
报告人简介：
Dr Yimin Chao BSc, PhD, MRSC, MInstP, FHEA
School of Chemistry
University of East Anglia
Norwich Research Park
Norwich NR4 7TJ
Tel: 01603 593146
Fax: 01603 592003
Email: y.chao@uea.ac.uk
1. Current Appointment:
Senior Lecturer in Nanosciences, School of Chemistry, University of East Anglia
2. Appointments
June/2007— RCUK Academic Fellow/Lecturer/Senior Lecturer in Nanosciences, School of Chemistry, University of East Anglia
Apr. /2004-June/2007 Research Fellow in Wolfson Chemical Nanoscience Lab, Newcastle University
Feb./2000-Jul/2000 IRC in Superconductivity, Cavendish Lab, University of Cambridge, Visiting Scholar,
Jan./1989-Feb./2000 Changzhou University
(June/1996-- Feb.2000 Director of Functional Materials Lab, Associate professor; June/1990-June/1996 Lecturer; Jan./1989-June/1990 Assistant lecturer)
July/1987-Jan./1989 Shandong University, Assistant lecturer
3. Education:
Jul. /2000-Jul./2004 School of Chemical Engineering and Advanced Materials, Newcastle University, PhD in nanotechnology
Sep./1985-Jul./1987 Physics Department, Peking University, MSc in Solid State Physics
Sep./1981-Jul./1985 Physics Department, Nanjing Normal University, BSc in Physics
4. Memberships of Professional Bodies
Royal Society of Chemistry, MRSC
Institute of Physics, MInstP
High Education Academy, FHEA
5. Teaching responsibility:
Module organizer for the following modules:
MF22 - Advanced Topics in Chemistry II;
1H26 - Elements of Chemical Physics;
1C0Y - Year 1 Chemistry Lab (Physical Chemistry part)
SCI7RD6Y - Nano-scale Sythesis and Characterisation
Other modules I am involved in:
ME1Y - Future Energy
2C2Y - Physical Chemistry I;
1H25 - Physics for Chemists;
M30Y - Advanced Lab;
3C0Y - BSc final year research project;
M40Y - MChem final year research project
6. Editorial memberships, experience in reviewing proposal/manuscripts:
Editorial board memberships: American Applied Sciences, Nanoscience and Nanotechnology
Reviewing proposals for funding bodies includes EPSRC, Leverhulme Trust, Saudi King Abdullah International Medical Research Center (KAIMRC)
Reviewing manuscripts for the following journals: Advanced Materials, Advanced Energy Materials, Advanced Functional Material, Angewandte Chemie, Small, Journal of Nanoparticle Research, Chemical Communication, Nanoscale, ACS Applied Materials & Interfaces, and Nanotechnology
7. Other administration role:
EPSRC Peer Review College member;
UEA China strategy board member;
School examination board member;
Associate director of UEA Energy Materials Laboratory
8. Research Profile:
Yimin Chao was appointed as a Senior Lecturer in the School of Chemistry at UEA on the basis of an established track record in investigating nanostructured systems from the basic physical and chemical mechanisms of synthesis, through their optical and electronic properties to scientific and industrial applications.1-5 Chao has a wide range of experiences in synthesis of nanoscale materials, electronic and optical spectroscopy and UHV techniques, using synchrotron and lab-based radiation to characterise novel nanoscale materials.
Various types of functionalized silicon quantum dots (SiQDs) have been developed in Chao lab with both top down and bottom up methods, including alkylated, poly-acrylic acid grafted, amine terminated, and sugar conjugated SiQDs. Most recent achievement is a bottom up method to produce SiQDs with various carbon chain lengths via micelle reduction. This method is providing a better pathway to control the size and functionalization of SiQDs.
Funding from the Royal Society/Wolfson Foundation and the University enabled the setting up of the UEA Energy Materials Laboratory of which Chao is Associate Director. He is a member of the EPSRC SolarCap consortium which is working on solar fuels, and a member of British Society for Nanomedicine.
Leading publications: (see webpage for full list since 2001)
(1) Chao, Y.; Šiller, L.; Krishnamurthy, S.; Coxon, P. R.; Bangert, U.; Gass, M.; Kjeldgaard, L.; Patoleo, S. N.; Lie, L. H.; O'Farrell, N.; Alsop, T. A.; Houlton, A.; Horrocks, B. R. Nat. Nanotechnol. 2007, 2, 486-489.
(2) Wang, Q.; Bao, Y.; Zhang, X.; Coxon, P. R.; Jayasooriya, U. A.; Chao, Y. Adv. Health. Mater. 2012, 1, 189-198.
(3) Alsharif, N. H.; Berger, C. E. M.; Varanasi, S. S.; Chao, Y.; Horrocks, B. R.; Datta, H. K. Small 2009, 5, 221-228.
(4) Ahire, J.; Wang, Q.; Coxon, P. R.; Malhotra, G.; Brydson, R. M. D.; Chen, R.; Chao, Y. ACS Appl. Mater. Interfaces 2012, 4, 3285-3292.
(5) Ashby, S.; Thomas, J.; García-Cañadas, J.; Min, G.; Corp, J.; Powell, A.; Xu, H.; Shen, W.; Chao, Y. Faraday Discuss.. 2014, 176, 349-361.
School of Chemistry
University of East Anglia
Norwich Research Park
Norwich NR4 7TJ
Tel: 01603 593146
Fax: 01603 592003
Email: y.chao@uea.ac.uk
1. Current Appointment:
Senior Lecturer in Nanosciences, School of Chemistry, University of East Anglia
2. Appointments
June/2007— RCUK Academic Fellow/Lecturer/Senior Lecturer in Nanosciences, School of Chemistry, University of East Anglia
Apr. /2004-June/2007 Research Fellow in Wolfson Chemical Nanoscience Lab, Newcastle University
Feb./2000-Jul/2000 IRC in Superconductivity, Cavendish Lab, University of Cambridge, Visiting Scholar,
Jan./1989-Feb./2000 Changzhou University
(June/1996-- Feb.2000 Director of Functional Materials Lab, Associate professor; June/1990-June/1996 Lecturer; Jan./1989-June/1990 Assistant lecturer)
July/1987-Jan./1989 Shandong University, Assistant lecturer
3. Education:
Jul. /2000-Jul./2004 School of Chemical Engineering and Advanced Materials, Newcastle University, PhD in nanotechnology
Sep./1985-Jul./1987 Physics Department, Peking University, MSc in Solid State Physics
Sep./1981-Jul./1985 Physics Department, Nanjing Normal University, BSc in Physics
4. Memberships of Professional Bodies
Royal Society of Chemistry, MRSC
Institute of Physics, MInstP
High Education Academy, FHEA
5. Teaching responsibility:
Module organizer for the following modules:
MF22 - Advanced Topics in Chemistry II;
1H26 - Elements of Chemical Physics;
1C0Y - Year 1 Chemistry Lab (Physical Chemistry part)
SCI7RD6Y - Nano-scale Sythesis and Characterisation
Other modules I am involved in:
ME1Y - Future Energy
2C2Y - Physical Chemistry I;
1H25 - Physics for Chemists;
M30Y - Advanced Lab;
3C0Y - BSc final year research project;
M40Y - MChem final year research project
6. Editorial memberships, experience in reviewing proposal/manuscripts:
Editorial board memberships: American Applied Sciences, Nanoscience and Nanotechnology
Reviewing proposals for funding bodies includes EPSRC, Leverhulme Trust, Saudi King Abdullah International Medical Research Center (KAIMRC)
Reviewing manuscripts for the following journals: Advanced Materials, Advanced Energy Materials, Advanced Functional Material, Angewandte Chemie, Small, Journal of Nanoparticle Research, Chemical Communication, Nanoscale, ACS Applied Materials & Interfaces, and Nanotechnology
7. Other administration role:
EPSRC Peer Review College member;
UEA China strategy board member;
School examination board member;
Associate director of UEA Energy Materials Laboratory
8. Research Profile:
Yimin Chao was appointed as a Senior Lecturer in the School of Chemistry at UEA on the basis of an established track record in investigating nanostructured systems from the basic physical and chemical mechanisms of synthesis, through their optical and electronic properties to scientific and industrial applications.1-5 Chao has a wide range of experiences in synthesis of nanoscale materials, electronic and optical spectroscopy and UHV techniques, using synchrotron and lab-based radiation to characterise novel nanoscale materials.
Various types of functionalized silicon quantum dots (SiQDs) have been developed in Chao lab with both top down and bottom up methods, including alkylated, poly-acrylic acid grafted, amine terminated, and sugar conjugated SiQDs. Most recent achievement is a bottom up method to produce SiQDs with various carbon chain lengths via micelle reduction. This method is providing a better pathway to control the size and functionalization of SiQDs.
Funding from the Royal Society/Wolfson Foundation and the University enabled the setting up of the UEA Energy Materials Laboratory of which Chao is Associate Director. He is a member of the EPSRC SolarCap consortium which is working on solar fuels, and a member of British Society for Nanomedicine.
Leading publications: (see webpage for full list since 2001)
(1) Chao, Y.; Šiller, L.; Krishnamurthy, S.; Coxon, P. R.; Bangert, U.; Gass, M.; Kjeldgaard, L.; Patoleo, S. N.; Lie, L. H.; O'Farrell, N.; Alsop, T. A.; Houlton, A.; Horrocks, B. R. Nat. Nanotechnol. 2007, 2, 486-489.
(2) Wang, Q.; Bao, Y.; Zhang, X.; Coxon, P. R.; Jayasooriya, U. A.; Chao, Y. Adv. Health. Mater. 2012, 1, 189-198.
(3) Alsharif, N. H.; Berger, C. E. M.; Varanasi, S. S.; Chao, Y.; Horrocks, B. R.; Datta, H. K. Small 2009, 5, 221-228.
(4) Ahire, J.; Wang, Q.; Coxon, P. R.; Malhotra, G.; Brydson, R. M. D.; Chen, R.; Chao, Y. ACS Appl. Mater. Interfaces 2012, 4, 3285-3292.
(5) Ashby, S.; Thomas, J.; García-Cañadas, J.; Min, G.; Corp, J.; Powell, A.; Xu, H.; Shen, W.; Chao, Y. Faraday Discuss.. 2014, 176, 349-361.
报告摘要：
Over the past decade, there has been an increasing interest in using nanotechnology for cancer therapy. The development of smart targeted nanoparticles (NPs) that can identify and deliver drugs at a sustained rate directly to cancer cells may provide better efficacy and lower toxicity for treating primary and advanced metastatic tumors. Obtaining knowledge of the diseases at the molecular level can facilitate the identification of biological targets. In particular, carbohydrate-mediated molecular recognitions using nano-vehicles are likely to increasingly affect cancer treatment methods, opening a new area in biomedical applications. Here, through accurate control over the surface functionalization of novel carbohydrate capped SiNPs by size distribution, absorption and an extensive physiochemical characterization, we correlated the cytotoxicity, stability and uptake of 6 nm Silicon NPs (SiNPs) coated with several types of biomolecules, including galactose, glucose, mannose and lactose. We noticed the extensive reduction in toxicity of SiNPs by functionalizing with carbohydrate moiety both in vitro and in vivo. Surprisingly, the carbohydrate capped SiNPs were found to be taken up more readily by cancer cells than non-cancerous cell types. Moreover, the NPs were internalized in the cells within 24 hours of incubation, as confirmed by flow-cytometry analysis. The uptake of carbohydrate capped SiNPs were visualized by fluorescence microscopy. These results reinforce the use of carbohydrates as an alternative to other conventional ligands such as PEG molecules for internalization into cancer cells.
